Chronic aspiration shifts the immune response from adaptive immunity to innate immunity in a murine model of asthma.
Inflamm Res
; 61(8): 863-73, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22565668
ABSTRACT
OBJECTIVE AND DESIGN:
The hypothesis that aspiration of gastric fluid drives the anti-ovalbumin response toward a Th2 reaction even in animals not prone to Th2 responses was evaluated.SUBJECTS:
Forty-eight male C57BL/6 mice were used.METHODS:
Mice were sensitized and challenged with ovalbumin starting 5 weeks prior to the initiation of weekly aspirations of either gastric fluid or normal saline as a control. Weekly aspiration continued during the course of exposure to ovalbumin. TREATMENT Aspiration consisted of 50 µl of gastric fluid with 50 µl of 0.9 % normal saline used as a control. Antigen exposure consisted of sensitization to ovalbumin via intraperitoneal injection on days 0 and 14 and challenge on day 21 with aerosolized antigen for 30 min.RESULTS:
No evidence of a shift toward a Th2 response as a result of gastric fluid aspiration was seen in the Th1-prone strain utilized, although a profound down-regulation of a broad array of T cell-associated cytokines and chemokines and up-regulation of macrophage-associated markers was observed as a result of aspiration.CONCLUSIONS:
These data provide support for the hypothesis that the clinical association between asthma and gastroesophageal reflux disease (GERD) does not involve an exacerbation of asthma by GERD-associated aspiration of gastric fluid, but may cause immune reactions unrelated to the asthma pathology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Asma
/
Aspiração Respiratória
/
Suco Gástrico
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article