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Metal-enhanced immunoassays.
Gryczynski, Ignacy; Luchowski, Rafal; Matveeva, Evgenia G; Shtoyko, Tanya; Sarkar, Pabak; Borejdo, Julian; Akopova, Irina; Gryczynski, Zygmunt.
Afiliação
  • Gryczynski I; Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, USA. igryczyn@hcs.unt.edu
Methods Mol Biol ; 875: 217-29, 2012.
Article em En | MEDLINE | ID: mdl-22573442
The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures--silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoensaio / Metais Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imunoensaio / Metais Idioma: En Ano de publicação: 2012 Tipo de documento: Article