Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma.
Invest Ophthalmol Vis Sci
; 53(7): 3847-57, 2012 Jun 22.
Article
em En
| MEDLINE
| ID: mdl-22589442
ABSTRACT
PURPOSE:
To study sequential changes in retinal ganglion cell (RGC) morphology in mice after optic nerve crush and after induction of experimental glaucoma.METHODS:
Nerve crush or experimental glaucoma was induced in mice that selectively express yellow fluorescent protein (YFP) in RGCs. Mice were euthanized 1, 4, and 9 days after crush and 1, 3, and 6 weeks after induction of glaucoma by bead injection. All YFP-RGCs were identified in retinal whole mounts. Then confocal images of randomly selected RGCs were quantified for somal fluorescence brightness, soma size, neurite outgrowth, and dendritic complexity (Sholl analysis).RESULTS:
By 9 days after crush, 98% of RGC axons died and YFP-RGCs decreased by 64%. After 6 weeks of glaucoma, 31% of axons died, but there was no loss of YFP-RGC bodies. All crush retinas combined had significant decreases in neurite outgrowth parameters (P ≤ 0.036, generalized estimating equation [GEE] model) and dendritic complexity was lower than controls (P = 0.017, GEE model). There was no change in RGC soma area after crush. In combined glaucoma data, the RGC soma area was larger than control (P = 0.04, GEE model). At 3 weeks, glaucoma RGCs had significantly larger values for dendritic structure and complexity than controls (P = 0.044, GEE model), but no statistical difference was found at 6 weeks.CONCLUSIONS:
After nerve crush, RGCs and axons died rapidly, and dendritic structure decreased moderately in remaining RGCs. Glaucoma caused an increase in RGC dendrite structure and soma size at 3 weeks.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células Ganglionares da Retina
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Axônios
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Glaucoma
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Apoptose
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Traumatismos do Nervo Óptico
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Modelos Animais de Doenças
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article