The protective effect of docosahexaenoic acid on the bilirubin neurotoxicity.
J Enzyme Inhib Med Chem
; 28(4): 801-7, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-22591320
ABSTRACT
Usually, all newborns demonstrate high serum unconjugated bilirubin (UCB) level. UCB may induce adverse effects in the central nervous system. We aimed to evaluate the cytotoxic effects of UCB and the protective effects of docosahexaenoic acid (DHA) on astrocyte cell cultures. The viability of astrocyte cells decreased after UCB treatment in a dose-dependent manner. Pre-incubation of DHA prevents the cells from UCB-mediated neurotoxicity. Our results shown that UCB leads to inhibition of antioxidant enzymes superoxide dismutase (SOD), catalase and GPx activity and induction of apoptosis. But only 4-h pretreatment of DHA can suppress of UCB-mediated inhibition of antioxidant enzymes SOD, catalase and GPx activity and induction of apoptosis in astrocytes. Our results strongly indicated that DHA has a protective effect on UCB-mediated neurotoxicity through inhibition apoptosis and antioxidant enzymes activity of SOD, CAT and GPx in rat primer astrocyte cell line.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bilirrubina
/
Ácidos Docosa-Hexaenoicos
/
Astrócitos
/
Apoptose
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article