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APELIN promotes hematopoiesis from human embryonic stem cells.
Yu, Qing C; Hirst, Claire E; Costa, Magdaline; Ng, Elizabeth S; Schiesser, Jacqueline V; Gertow, Karin; Stanley, Edouard G; Elefanty, Andrew G.
Afiliação
  • Yu QC; Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Australia.
Blood ; 119(26): 6243-54, 2012 Jun 28.
Article em En | MEDLINE | ID: mdl-22611158
Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold. Furthermore, APLN peptide also synergized with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC-derived hematopoietic and endothelial cells.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos e Proteínas de Sinalização Intercelular / Células-Tronco Embrionárias / Hematopoese Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos e Proteínas de Sinalização Intercelular / Células-Tronco Embrionárias / Hematopoese Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article