Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine.
Bioorg Med Chem Lett
; 22(12): 3870-2, 2012 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-22613261
ABSTRACT
The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.
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Base de dados:
MEDLINE
Assunto principal:
Oligorribonucleotídeos
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Terminação Traducional da Cadeia Peptídica
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Purinas
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Biossíntese de Proteínas
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Compostos de Vinila
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RNA Mensageiro
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article