Revisiting channel allostery: a coherent mechanism in IP3 and ryanodine receptors.
Sci Signal
; 5(225): pe24, 2012 May 22.
Article
em En
| MEDLINE
| ID: mdl-22623752
ABSTRACT
The inositol 1,4,5-trisphosphate (IP3) receptor is an IP3-gated calcium ion (Ca²âº) channel that mediates intracellular IP3-Ca²âº signaling. A fundamental question--how IP3 gates the Ca²âº channel within the IP3 receptor--remains unanswered. A new crystal structure of the N-terminal region of the IP3 receptor reveals allosteric changes by ligand binding and its similarity to the corresponding region of ryanodine receptor. Docking of the crystal structures in the electron microscopy map and an IP3 receptor-ryanodine receptor chimera consistently supported a coherent gating mechanism in these receptors. An intriguing feature was the long distance between the IP3-binding sites and the Ca²âº channel, suggesting that long-range allosteric coupling occurs between these regions upon gating of the channel. These results help integrate previous knowledge on the IP3 and ryanodine receptors and also provide a new framework for understanding the gating mechanism.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ativação do Canal Iônico
/
Canal de Liberação de Cálcio do Receptor de Rianodina
/
Sinalização do Cálcio
/
Receptores de Inositol 1,4,5-Trifosfato
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article