Tobacco calmodulin-like protein provides secondary defense by binding to and directing degradation of virus RNA silencing suppressors.
Proc Natl Acad Sci U S A
; 109(25): 10113-8, 2012 Jun 19.
Article
em En
| MEDLINE
| ID: mdl-22665793
ABSTRACT
RNA silencing (RNAi) induced by virus-derived double-stranded RNA (dsRNA), which is in a sense regarded as a pathogen-associated molecular pattern (PAMP) of viruses, is a general plant defense mechanism. To counteract this defense, plant viruses express RNA silencing suppressors (RSSs), many of which bind to dsRNA and attenuate RNAi. We showed that the tobacco calmodulin-like protein, rgs-CaM, counterattacked viral RSSs by binding to their dsRNA-binding domains and sequestering them from inhibiting RNAi. Autophagy-like protein degradation seemed to operate to degrade RSSs with the sacrifice of rgs-CaM. These RSSs could thus be regarded as secondary viral PAMPs. This study uncovered a unique defense system in which an rgs-CaM-mediated countermeasure against viral RSSs enhanced host antiviral RNAi in tobacco.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
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Nicotiana
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RNA Viral
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Inativação Gênica
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article