Galacto-oligosaccharide synthesis from lactose solution or skim milk using the ß-galactosidase from Bacillus circulans.
J Agric Food Chem
; 60(25): 6391-8, 2012 Jun 27.
Article
em En
| MEDLINE
| ID: mdl-22676418
ABSTRACT
The synthesis of galacto-oligosaccharides (GOS) catalyzed by a novel commercial preparation of ß-galactosidase from Bacillus circulans (Biolactase) was studied, and the products were characterized by MS and NMR. Using 400 g/L lactose and 1.5 enzyme units per milliliter, the maximum GOS yield, measured by HPAEC-PAD analysis, was 165 g/L (41% w/w of total carbohydrates in the mixture). The major transgalactosylation products were the trisaccharide Gal-ß(1â4)-Gal-ß(1â4)-Glc and the tetrasaccharide Gal-ß(1â4)-Gal-ß(1â4)-Gal-ß(1â4)-Glc. The GOS yield increased to 198 g/L (49.4% w/w of total carbohydrates) using a higher enzyme concentration (15 U/mL), which minimized the enzyme inactivation under reaction conditions. Using skim milk (with a lactose concentration of 46 g/L), the enzyme also displayed transgalactosylation activity maximum GOS yield accounted for 15.4% (7.1 g/L), which was obtained at 50% lactose conversion.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
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Bacillus
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Proteínas Fúngicas
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Beta-Galactosidase
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Leite
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Lactose
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article