Low-resolution structure determination of Na(+)-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy.
Acta Crystallogr D Biol Crystallogr
; 68(Pt 6): 724-31, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22683795
ABSTRACT
A low-resolution structure of the Na(+)-translocating NADHubiquinone oxidoreductase from the human pathogen Vibrio cholerae was determined by ab initio phasing and independently confirmed by electron microscopy. This multi-subunit membrane-protein complex (molecular weight 210 kDa) generates an Na(+) gradient that is essential for substrate uptake, motility, pathogenicity and efflux of antibiotics. The obtained 16 Å resolution electron density-map revealed an asymmetric particle with a central region of low electron density and a putative detergent region, and allowed the identification of the transmembrane regions of the complex.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vibrio cholerae
/
Complexo I de Transporte de Elétrons
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article