New meta-analysis tools reveal common transcriptional regulatory basis for multiple determinants of behavior.
Proc Natl Acad Sci U S A
; 109(26): E1801-10, 2012 Jun 26.
Article
em En
| MEDLINE
| ID: mdl-22691501
ABSTRACT
A fundamental problem in meta-analysis is how to systematically combine information from multiple statistical tests to rigorously evaluate a single overarching hypothesis. This problem occurs in systems biology when attempting to map genomic attributes to complex phenotypes such as behavior. Behavior and other complex phenotypes are influenced by intrinsic and environmental determinants that act on the transcriptome, but little is known about how these determinants interact at the molecular level. We developed an informatic technique that identifies statistically significant meta-associations between gene expression patterns and transcription factor combinations. Deploying this technique for brain transcriptome profiles from ca. 400 individual bees, we show that diverse determinants of behavior rely on shared combinations of transcription factors. These relationships were revealed only when we considered complex and variable regulatory rules, suggesting that these shared transcription factors are used in distinct ways by different determinants. This regulatory code would have been missed by traditional gene coexpression or cis-regulatory analytic methods. We expect that our meta-analysis tools will be useful for a broad array of problems in systems biology and other fields.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Comportamento Animal
/
Metanálise como Assunto
Tipo de estudo:
Prognostic_studies
/
Systematic_reviews
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article