α-cyperone alleviates lung cell injury caused by Staphylococcus aureus via attenuation of α-hemolysin expression.
J Microbiol Biotechnol
; 22(8): 1170-6, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22713997
ABSTRACT
In this study, we aimed to evaluate the effect of α- cyperone on S. aureus. We used a hemolysin test to examine the hemolytic activity in supernatants of S. aureus cultured with increasing concentrations of α- cyperone. In addition, we evaluated the production of α- hemolysin (Hla) by Western blotting. Real-time RT-PCR was performed to test the expression of hla (the gene encoding Hla) and agr (accessory gene regulator). Furthermore, we investigated the protective effect of α- cyperone on Hla-induced injury of A549 lung cells by live/ dead and cytotoxicity assays. We showed that in the presence of subinhibitory concentrations of α-cyperone, Hla production was markedly inhibited. Moreover, α- cyperone protected lung cells from Hla-induced injury. These findings indicate that α-cyperone is a promising inhibitor of Hla production by S. aureus and protects lung cells from this bacterium. Thus, α-cyperone may provide the basis for a new strategy to combat S. aureus pneumonia.
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Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
Toxinas Bacterianas
/
Expressão Gênica
/
Fatores de Virulência
/
Proteínas Hemolisinas
/
Antibacterianos
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article