[Effects of ABA and its biosynthetic inhibitor fluridone on accumulation of penolic acids and activity of PAL and TAT in hairy root of Salvia miltiorrhiza].

ABSTRACT

OBJECTIVE: To study the function of ABA and fluridone on the contents of penolic acids and two key synthetases (PAL and TAT). METHOD: Conducted 4 different concentrations in the hairy root of Salvia miltiorrhiza after culturing 18 days and treated with fluridone. One day later, harvested the hairy root and measured the activity of PAL and TAT; Treatment for 6 days, gathered and determined the contents of phenolic acids. RESULT: In certain concentration of ABA, lower ABA could induced the production of growth and higher ABA inhibitor the growth in hairy roots of S. miltiorrhiza; ABA induced the accumulation of caffeic acid considerably, and the effect on the contents of coffee acid show positive correlation; As for the RA and LAB, the low dosage of ABA simulated the production and higher ABA inhibited the production of them; the ABA biosynthetic inhibitor fluridone can decreases ABA's the effect; The different of ABA activated the activity of PAL and TAT, but the impact were discriminating, when treatment with ABA and fluridone, the inducing were declined. CONCLUSION: ABA induced the accumulation of.