In planta production of a candidate vaccine against bovine papillomavirus type 1.
Planta
; 236(4): 1305-13, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22718313
ABSTRACT
Bovine papillomavirus type 1 (BPV-1) is an economically important virus that induces tumourigenic pathologies in horses and cows. Given that the BPV-1 L1 major coat protein can self-assemble into highly immunogenic higher-order structures, we transiently expressed it in Nicotiana benthamiana as a prelude to producing a candidate vaccine. It was found that plant codon optimization of L1 gave higher levels of expression than its non-optimized counterpart. Following protein extraction, we obtained high yields (183 mg/kg fresh weight leaf tissue) of relatively pure L1, which had self-assembled into virus-like particles (VLPs). We found that these VLPs elicited a highly specific and strong immune response, and therefore they may have utility as a potential vaccine. This is the first report demonstrating the viable production of a candidate BPV vaccine protein in plants.
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Base de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Proteínas do Capsídeo
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Papillomavirus Bovino 1
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Vacinas de Partículas Semelhantes a Vírus
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Anticorpos Antivirais
/
Antígenos Virais
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article