Methods for chromatographic removal of endotoxin.
Methods Mol Biol
; 899: 265-75, 2012.
Article
em En
| MEDLINE
| ID: mdl-22735959
ABSTRACT
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Lipopolissacarídeos
/
Cromatografia Líquida
/
Endotoxinas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article