Scanning angle interference microscopy reveals cell dynamics at the nanoscale.
Nat Methods
; 9(8): 825-7, 2012 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-22751201
ABSTRACT
Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto
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Membrana Celular
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Nanotecnologia
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Células Epiteliais
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Microscopia de Interferência
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article