Polyethylene glycol-mediated transformation of fused egfp-hph gene under the control of gpd promoter in Pleurotus eryngii.
Biotechnol Lett
; 34(10): 1895-900, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22763850
Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the control of the strong and constitutive native gpd promoter from P. eryngii. The recombinant plasmid was used to transform of P. eryngii protoplasts. Successful transformation was demonstrated by molecular analyses. Moreover, the mycelia of the transformants showed green epipolic dispersion on fluorescence microscopy. About 90-210 transformants were produced per µg plasmid DNA per 10(7) viable protoplasts.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
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Proteínas Recombinantes de Fusão
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Transfecção
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Pleurotus
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Proteínas de Fluorescência Verde
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article