VEGF microsphere technology to enhance vascularization in fat grafting.
Ann Plast Surg
; 69(2): 213-9, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22772067
ABSTRACT
Vascularization is crucial for implantation of engineered tissues in reconstructive surgery. Polypeptides encapsulated in microspheres can be efficiently transported to their site of action and released in a sustained dosage. We evaluated the effect of delivering vascular endothelial growth factor (VEGF)-encapsulated microspheres in a lipoaspirate scaffold on vascularization and tissue survival. The VEGF-loaded (n=6) and empty (n=6) poly(lactic-co-glycolic acid) microspheres in human lipoaspirate and the human lipoaspirate alone (n=6) were injected subcutaneously into the flanks of athymic nude mice. Three mice from each group were killed, and grafts were explanted at weeks 3 and 6. Increases in mass and volume of VEGF samples, as well as decreases in empty and lipoaspirate-only samples, were observed at 3 and 6 weeks, reaching statistical significance at 6 weeks. Hematoxylin and eosin and CD31+ imaging demonstrated significantly greater vascularization in VEGF samples than in both the empty and lipoaspirate-only groups at both 3 and 6 weeks.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neovascularização Fisiológica
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Fator A de Crescimento do Endotélio Vascular
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Indutores da Angiogênese
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Regeneração Tecidual Guiada
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Tecido Adiposo Branco
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Alicerces Teciduais
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Microesferas
Tipo de estudo:
Evaluation_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article