Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups.
J Clin Microbiol
; 50(9): 3108-10, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22785193
ABSTRACT
Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Bacteriológicas
/
Técnicas de Diagnóstico Molecular
/
Escherichia coli
/
Infecções por Escherichia coli
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article