Interchenar retrotransfer of aureothin intermediates in an iterative polyketide synthase module.
J Am Chem Soc
; 134(30): 12382-5, 2012 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-22799266
ABSTRACT
The course of the enigmatic iterative use of a polyketide synthase module was deduced from targeted domain inactivation in the aureothin assembly line. Mutational analyses revealed that the N-terminus of AurA is not involved in the iteration process, ruling out an ACP-ACP shuttle. Furthermore, an AurA(KS°, ACP°)-AurA(AT(0)) heterodimer proved to be nonfunctional, whereas aureothin production was restored in a ΔaurA mutant complemented with AurA(KS°)-AurA(ACP°). This finding supports a model according to which the ACP-bound polyketide intermediate is transferred back to the KS domain on the opposite PKS strand.
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1
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Cromonas
/
Policetídeo Sintases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article