Cytokine responses to novel antigens in a peri-urban population in Brazil exposed to Leishmania infantum chagasi.
Am J Trop Med Hyg
; 87(4): 663-70, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22826477
ABSTRACT
Visceral leishmaniasis (VL) is fatal if untreated, and there are no vaccines for this disease. High levels of CD4-derived interferon-γ (IFN-γ) in the presence of low levels of interleukin-10 (IL-10) predicts vaccine success. Tumor necrosis factor-α (TNF-α) is also important in this process. We characterized human immune responses in three groups exposed to Leishmania infantum chagasi in Brazil 1) drug-cured VL patients (recovered VL); 2) asymptomatic persons with positive Leishmania-specific delayed-type hypersensitivity skin reactions (DTH+); and 3) DTH-negative household contacts. Magnitude of DTH correlated with crude Leishmania antigen-driven IFN-γ, TNF-α, and IL-5, but not IL-10. DTH+ persons showed equivalent levels of IFN-γ, but higher levels of IL-10, to tryparedoxin peroxidase and Leishmania homolog of receptor for activated C kinase compared with recovered VL patients. The IFN-γIL-10 and TNF-αIL-10 ratios were higher in recovered VL patients than in DTH+ persons. Seven of 11 novel candidates (R71, L37, N52, L302.06, M18, J41, and M22) elicited cytokine responses (36-71% of responders) in recovered VL patients and DTH+ persons. This result confirmed their putative status as cross-species vaccine/immunotherapeutic candidates.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
População Urbana
/
Citocinas
/
Leishmania infantum
/
Leishmaniose Visceral
/
Antígenos de Protozoários
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article