Biological tissue imaging with a position and time sensitive pixelated detector.
J Am Soc Mass Spectrom
; 23(10): 1679-88, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22836864
ABSTRACT
We demonstrate the capabilities of a highly parallel, active pixel detector for large-area, mass spectrometric imaging of biological tissue sections. A bare Timepix assembly (512 × 512 pixels) is combined with chevron microchannel plates on an ion microscope matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI TOF-MS). The detector assembly registers position- and time-resolved images of multiple m/z species in every measurement frame. We prove the applicability of the detection system to biomolecular mass spectrometry imaging on biologically relevant samples by mass-resolved images from Timepix measurements of a peptide-grid benchmark sample and mouse testis tissue slices. Mass-spectral and localization information of analytes at physiologic concentrations are measured in MALDI-TOF-MS imaging experiments. We show a high spatial resolution (pixel size down to 740 × 740 nm(2) on the sample surface) and a spatial resolving power of 6 µm with a microscope mode laser field of view of 100-335 µm. Automated, large-area imaging is demonstrated and the Timepix' potential for fast, large-area image acquisition is highlighted.
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Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Imagem Molecular
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article