A central coupler for recombination initiation linking chromosome architecture to S phase checkpoint.
Mol Cell
; 47(5): 722-33, 2012 Sep 14.
Article
em En
| MEDLINE
| ID: mdl-22841486
ABSTRACT
Higher-order chromosome structure is assumed to control various DNA-templated reactions in eukaryotes. Meiotic chromosomes implement developed structures called "axes" and "loops"; both are suggested to tether each other, activating Spo11 to catalyze meiotic DNA double-strand breaks (DSBs) at recombination hotspots. We found that the Schizosaccharomyces pombe Spo11 homolog Rec12 and its partners form two distinct subcomplexes, DSBC (Rec6-Rec12-Rec14) and SFT (Rec7-Rec15-Rec24). Mde2, whose expression is strictly regulated by the replication checkpoint, interacts with Rec15 to stabilize the SFT subcomplex and further binds Rec14 in DSBC. Rec10 provides a docking platform for SFT binding to axes and can partially interact with DSB sites located in loops depending upon Mde2, which is indicative of the formation of multiprotein-based tethered axis-loop complex. These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint.
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Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Schizosaccharomyces
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Fase S
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Cromossomos
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Proteínas de Schizosaccharomyces pombe
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Endodesoxirribonucleases
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Fatores de Transcrição Forkhead
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article