Human microparticles generated during sepsis in patients with critical illness are neutrophil-derived and modulate the immune response.
J Trauma Acute Care Surg
; 73(2): 401-6; discussion 406-7, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22846946
ABSTRACT
BACKGROUND:
Microparticles (MPs) are 0.3 µm to 1.0 µm vesicles generated after cell activation or apoptosis that may play a role in the pathophysiology of sepsis. We sought to elucidate the role of MPs in patients with critical illness and hypothesized that MPs are generated at the site of inflammation and can modulate the immune response.METHODS:
Surgical patients with critical illness with ongoing sepsis were enrolled from the intensive care unit of an urban, Level I trauma center from March to June 2011. Abdominal washings and bronchoalveolar lavage fluid were collected from sites of inflammation. MPs were isolated using differential centrifugation, then characterized by flow cytometry. Immunologic assays were conducted by incubating neutrophil-derived MPs (NDMPs) with a human monocytic cell line (THP-1). A p value ≤0.05 was considered significant.RESULTS:
MPs were absent in noninflamed foci in patients, whereas NDMPs were present in locations of inflammation. NDMPs were added to cultured THP-1 cells to quantify immunomodulatory effects. THP-1 cells were able to phagocytose NDMPs. Cells that ingested NDMPs demonstrated increased activation. In contrast, bystander THP-1 cells without ingested NDMPs demonstrated decreased activation.CONCLUSION:
NDMPs are generated at the site of inflammation in patients with critical illness during sepsis. They have a divergent effect on the immune response by activating phagocytic cells and deactivating bystander cells. NDMPs may play an important role in regulating the inflammatory response to sepsis in patients with critical illness.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Sepse
/
Micropartículas Derivadas de Células
/
Neutrófilos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adult
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article