The first aptamer-apheresis column specifically for clearing blood of ß1-receptor autoantibodies.
Circ J
; 76(10): 2449-55, 2012.
Article
em En
| MEDLINE
| ID: mdl-22850243
ABSTRACT
BACKGROUND:
Application of immunoapheresis to eliminate pathogenic autoantibodies targeting the second extracellular loop of the ß1-receptor (ß1-AABs) is currently investigated in patients with cardiomyopathy. Aptamers (single short DNA or RNA strands) are a new class of molecules that bind to a specific target molecule. This property qualifies aptamers for potential use in the apheresis technique. We recently identified an aptamer that specifically binds to ß1-AABs, so in the present study we tested whether this aptamer could be used as a binder to prepare an apheresis column suitable for clearing ß1-AABs from rat's blood. METHODS ANDRESULTS:
An apheresis column was designed containing the ß1-AAB-targeting-aptamer coupled to sepharose. As tested in vitro, this column (1) binds ß1-AABs highly specifically without marked interference with common IgGs, (2) has a capacity for clearing of approximately 1L of ß1-AAB-positive serum and (3) can be completely regenerated for subsequent use. Using the column for extracorporeal apheresis of spontaneously hypertensive rats (SHR) positive for both ß1-AABs and muscarinic 2-receptor autoantibodies (M2-AABs), only ß1-AABs were removed. In a follow-up of 9 weeks, recurrence of ß1-AABs in the blood of SHR could not be detected.CONCLUSIONS:
For the first time, a newly designed apheresis column with a ß1-AAB specific aptamer as a binder was successfully used to eliminate ß1-AABs from SHR blood.
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Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
/
Remoção de Componentes Sanguíneos
/
Receptores Adrenérgicos beta 1
/
Aptâmeros de Nucleotídeos
/
Imunoadsorventes
/
Cardiomiopatias
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article