Purification and characterization of a new bacteriocin active against Campylobacter produced by Lactobacillus salivarius SMXD51.
Food Microbiol
; 32(1): 129-34, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22850384
ABSTRACT
Strain SMXD51, isolated from chicken ceca and identified as Lactobacillus salivarius, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria and especially Campylobacter jejuni. The active peptide from the cell-free supernatant of Lb. salivarius SMXD51 was purified in three steps:
(i) precipitation with 80% saturated ammonium sulfate, (ii) elution on a reversed phase SPE UPTI-CLEAN cartridge using different concentrations of acetonitrile, (iii) final purification by reversed phase HPLC on a C(18) column. The mode of action of this peptide of 5383.2 Da was identified as bactericidal, and its amino acid composition was established. This new bacteriocin SMXD51 appears potentially very useful to reduce Campylobacter in poultry prior to processing.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bacteriocinas
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Campylobacter jejuni
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Lactobacillus
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article