A short polybasic segment between the two conserved domains of the ß2a-subunit modulates the rate of inactivation of R-type calcium channel.
J Biol Chem
; 287(39): 32588-97, 2012 Sep 21.
Article
em En
| MEDLINE
| ID: mdl-22851179
ABSTRACT
Besides opening and closing, high voltage-activated calcium channels transit to a nonconducting inactivated state from which they do not re-open unless the plasma membrane is repolarized. Inactivation is critical for temporal regulation of intracellular calcium signaling and prevention of a deleterious rise in calcium concentration. R-type high voltage-activated channels inactivate fully in a few hundred milliseconds when expressed alone. However, when co-expressed with a particular ß-subunit isoform, ß(2a), inactivation is partial and develops in several seconds. Palmitoylation of a unique di-cysteine motif at the N terminus anchors ß(2a) to the plasma membrane. The current view is that membrane-anchored ß(2a) immobilizes the channel inactivation machinery and confers slow inactivation phenotype. ß-Subunits contain one Src homology 3 and one guanylate kinase domain, flanked by variable regions with unknown structures. Here, we identified a short polybasic segment at the boundary of the guanylate kinase domain that slows down channel inactivation without relocating a palmitoylation-deficient ß(2a) to the plasma membrane. Substitution of the positively charged residues within this segment by alanine abolishes its slow inactivation-conferring phenotype. The linker upstream from the polybasic segment, but not the N- and C-terminal variable regions, masks the effect of this determinant. These results reveal a novel mechanism for inhibiting voltage-dependent inactivation of R-type calcium channels by the ß(2a)-subunit that might involve electrostatic interactions with an unknown target on the channel's inactivation machinery or its modulatory components. They also suggest that intralinker interactions occlude the action of the polybasic segment and that its functional availability is regulated by the palmitoylated state of the ß(2a)-subunit.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ativação do Canal Iônico
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Canais de Cálcio Tipo R
/
Subunidades Proteicas
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Lipoilação
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article