A modified simple method for determination of serum α-tocopherol (vitamin E).
J Basic Clin Physiol Pharmacol
; 23(1): 45-8, 2012 Jan 19.
Article
em En
| MEDLINE
| ID: mdl-22865449
ABSTRACT
BACKGROUND:
Vitamin E is one of the important antioxidants linked to regulate various diseases, such as atherosclerosis, hypertension, and male infertility. A relatively simple and economic biochemical modified method has been developed to determine serum α-tocopherol concentration.METHODS:
The current modified method is based on previous Baker and Frank method and the method of Martinek by using 2,2'-bipyridyl, ferric chloride, and xylene. The complex of ferrous ions generated in this reaction with 2,2'-bipyridyl is determined by using a plain enzyme-linked immunosorbent assay microplate (non-antibody coated) at 492 nm.RESULTS:
The standard curve of this new modified method shows a linearity with correlation r=0.997 (concentration vs. absorbance). The absorbance of this color complex is directly proportional to the α-tocopherol concentration. The sensitivity of this new modified method has been compared and correlated with Baker and Frank method by using 15 human samples (r=0.99, p<0.0001).CONCLUSIONS:
This simple and economic method may be routinely used to analyze α-tocopherol concentration in serum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vitamina E
/
Ensaio de Imunoadsorção Enzimática
/
Antioxidantes
Limite:
Adolescent
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Adult
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Humans
/
Middle aged
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article