Identification of the HIV-1 NC binding interface in Alix Bro1 reveals a role for RNA.
J Virol
; 86(21): 11608-15, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-22896625
ABSTRACT
HIV-1 recruits members of ESCRT, the cell membrane fission machinery that promotes virus exit. HIV-1 Gag protein gains access to ESCRT directly by binding Alix, an ESCRT-associated protein that promotes budding. The Alix Bro1 and V domains bind Gag NC and p6 regions, respectively. Whereas V-p6 binding and function are well characterized, residues in Bro1 that interact with NC and their functional contribution to Alix-mediated HIV-1 budding are unknown. We mapped Bro1 residues that constitute the NC binding interface and found that they are critical for function. Intriguingly, residues involved in interactions on both sides of the Bro1-NC interface are positively charged, suggesting the involvement of a negatively charged cellular factor serving as a bridge. Nuclease treatment eliminated Bro1-NC interactions, revealing the involvement of RNA. These findings establish a direct role for NC in mediating interactions with ESCRT necessary for virus release and report the first evidence of RNA involvement in such recruitments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao Cálcio
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RNA Viral
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Proteínas de Ciclo Celular
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Produtos do Gene gag do Vírus da Imunodeficiência Humana
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Interações Hospedeiro-Patógeno
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Domínios e Motivos de Interação entre Proteínas
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Complexos Endossomais de Distribuição Requeridos para Transporte
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article