Preparation of mineralized tissue for light microscopy.

ABSTRACT

Production of stained tissue sections for examination by light microscopy is a step-wise process which begins with preservation of tissue (fixation), then dehydration and clearing of the tissue, and finally impregnation with wax (processing). Mineralized tissues such as bone and teeth are subject to a further step (decalcification). Thin sections are then attached to a glass slide for staining and light microscopy.In the UK, it is usual for a preliminary diagnosis to be made using hematoxylin and eosin (H&E) staining. A definitive diagnosis may need further investigation with immunocytochemistry. All of these procedures must allow morphology and tissue structure to remain in tact, as any pathology present must not be compromised.