Segmenting data sets for RIP.
Acta Crystallogr D Biol Crystallogr
; 68(Pt 9): 1152-62, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22948916
ABSTRACT
Specific radiation damage can be used for the phasing of macromolecular crystal structures. In practice, however, the optimization of the X-ray dose used to `burn' the crystal to induce specific damage can be difficult. Here, a method is presented in which a single large data set that has not been optimized in any way for radiation-damage-induced phasing (RIP) is segmented into multiple sub-data sets, which can then be used for RIP. The efficacy of this method is demonstrated using two model systems and two test systems. A method to improve the success of this type of phasing experiment by varying the composition of the two sub-data sets with respect to their separation by image number, and hence by absorbed dose, as well as their individual completeness is illustrated.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Cristalografia por Raios X
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article