Cloning and genomic nucleotide sequence of the matrix attachment region binding protein from the halotolerant alga Dunaliella salina.
J Basic Microbiol
; 53(7): 622-9, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-22961592
ABSTRACT
In our previous study, the sequence of a matrix attachment region binding protein (MBP) cDNA was cloned from the unicellular green alga Dunaliella salina. However, the nucleotide sequence of this gene has not been reported so far. In this paper, the nucleotide sequence of MBP was cloned and characterized, and its gene copy number was determined. The MBP nucleotide sequence is 5641 bp long, and interrupted by 12 introns ranging from 132 to 562 bp. All the introns in the D. salina MBP gene have orthodox splice sites, exhibiting GT at the 5' end and AG at the 3' end. Southern blot analysis showed that MBP only has one copy in the D. salina genome.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Proteínas de Ligação à Região de Interação com a Matriz
/
Clorófitas
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article