Activation of GABA(A) receptors suppresses ethanol-induced upregulation of type 1 IP(3) receptors.
Synapse
; 67(1): 51-5, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23015304
ABSTRACT
Although Type 1 inositol 1,4,5-trisphosphate receptors (IP(3) Rs-1) are one of the major calcium channels to regulate intracellular Ca(2+) concentration, there have been few available data how their expression is modified by long-term exposure to ethanol. The present study attempted to clarify mechanisms of modification of IP(3) R-1 expression during long-term ethanol exposure by γ-aminobutyric acid (GABA)A receptors using mouse cerebral cortical neurons. Long-term exposure to ethanol induced IP(3) R-1 protein upregulation following increased expression of its mRNA. Pretreatment with muscimol, a selective GABA(A) receptor agonist, significantly suppressed the ethanol-induced upregulation of IP(3) R-1 protein and its mRNA, which was significantly abolished by bicuculline, a selective GABA(A) receptor antagonist. These results indicate that GABA(A) receptors negatively regulate the ethanol-induced upregulation of IP(3) R-1 protein expression via the suppression of gene transcription.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Regulação para Cima
/
Receptores de GABA-A
/
Etanol
/
Receptores de Inositol 1,4,5-Trifosfato
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article