[Protection and mechanism of Fagopyrum cymosum on lung injury in rats with Klebsiella pneumonia].
Zhong Yao Cai
; 35(4): 603-7, 2012 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-23019909
ABSTRACT
OBJECTIVE:
To study the mechanism of protective effect of Fagopyrum cymosum on lung injury induced by Klebsiella pneumonia in rats.METHODS:
The model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, Fagopyrum cymosum (6, 3, 1.5 g/kg) three groups, levofloxacin (25 mg/kg) group. The pathological change of lung was observed. The content of IL-1beta, IL-6, IL-8, TNF-alpha, ICAM-1, INF-gamma in serum were measured by radioimmunoassay and Elisa. TNF-alpha, ICAM-1, NF-kappaB p65 protein expressions were measured by immunohistochemistry. MIP-2mRNA expression was detected by in situ hybridization.RESULTS:
The rats of model group had obvious lung injury, but those of Fagopyrum cymosum and levofloxacin groups had less injury. The contents of IL-1beta, IL-6, IL-,8, TNF-alpha, ICAM-1 and INF-gamma in serum and the expressions of TNF-a, ICAM-1, NF-kappaB p65 and MIP--2mRNA of model group were significantly higher than those of the control group (P < 0.05 or P < 0.01), while the indexes of Fagopyrum cymosum and levofloxacin groups were significantly lower than those of model group (P < 0.05 or P < 0.01).CONCLUSION:
The lung injury induced by Klebsiella pneumonia is related to TNF-alpha, ICAM-1, NF-kappaB p65 and MIP-2mRNA. To decrease the excessive expression of TNF-alpha, ICAM-1, NF-kappaB p65 and MIP-2mRNA might be the main mechanism of protective effect of Fagopyrum cymosum on lung injury.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Infecções por Klebsiella
/
Medicamentos de Ervas Chinesas
/
Citocinas
/
Pneumonia Bacteriana
/
Fagopyrum
/
Pulmão
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article