Secreted Vago restricts West Nile virus infection in Culex mosquito cells by activating the Jak-STAT pathway.
Proc Natl Acad Sci U S A
; 109(46): 18915-20, 2012 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-23027947
ABSTRACT
Although West Nile virus (WNV) and other arthropod-borne viruses are a major public health problem, the mechanisms of antiviral immunity in mosquitoes are poorly understood. Dicer-2, responsible for the RNAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box helicase domain similar to mammalian RIG-I/MDA5 which, in Drosophila, was found to be required for activation of an antiviral gene, Vago. Here we show that the Culex orthologue of Vago (CxVago) is up-regulated in response to WNV infection in a Dicer-2-dependent manner. Further, our data show that CxVago is a secreted peptide that restricts WNV infection by activation of the Jak-STAT pathway. Thus, Vago appears to function as an IFN-like antiviral cytokine in mosquitoes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus do Nilo Ocidental
/
Citocinas
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Proteínas de Insetos
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Culex
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Fatores de Transcrição STAT
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Janus Quinases
/
Imunidade Inata
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article