Recovery of Bordetella pertussis from PCR-positive nasopharyngeal samples is dependent on bacterial load.
J Clin Microbiol
; 50(12): 4114-5, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23035189
ABSTRACT
Viable Bordetella pertussis isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples. B. pertussis was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover B. pertussis isolates, and culture can be limited to samples with a high bacterial load.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bordetella pertussis
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Coqueluche
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Nasofaringe
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Reação em Cadeia da Polimerase
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Técnicas Bacteriológicas
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Carga Bacteriana
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
Limite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article