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Towards a non-living vaccine against Shigella flexneri: from the inactivation procedure to protection studies.
Camacho, A I; Souza-Rebouças, J; Irache, J M; Gamazo, C.
Afiliação
  • Camacho AI; Department of Microbiology, University of Navarra, 31008 Pamplona, Spain.
Methods ; 60(3): 264-8, 2013 May 01.
Article em En | MEDLINE | ID: mdl-23046911
Shigellosis is one of the leading causes of diarrhea worldwide with more than 165 million cases annually. Hence, a vaccine against this disease is a priority, but no licensed vaccine is still available. Considering target population as well as intrinsic risks of live attenuated vaccines, non-living strategies appear as the most promising candidates. Remarkably, the preservation of antigenic properties is a major concern since inactivation methods of bacteria affect these qualities. We previously reported the use of a subcellular antigen complex for vaccination against shigellosis, based on outer membrane vesicles (OMVs) released from Shigella flexneri. Now, we describe in more detail the employment of binary ethylenimine (BEI) for inactivation of Shigella and its subsequent effect on the antigenic conservation of the vaccinal product. Results demonstrate the effectiveness of BEI treatment to completely inactivate Shigella cells without disturbing the antigenicity and immunogenicity of the OMVs. Thus, OMVs harvested after BEI inactivation were able to protect mice against an experimental infection with S. flexneri.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Shigella flexneri / Aziridinas / Proteínas da Membrana Bacteriana Externa / Vacinas contra Shigella / Disenteria Bacilar / Antígenos de Bactérias Limite: Animals Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Shigella flexneri / Aziridinas / Proteínas da Membrana Bacteriana Externa / Vacinas contra Shigella / Disenteria Bacilar / Antígenos de Bactérias Limite: Animals Idioma: En Ano de publicação: 2013 Tipo de documento: Article