A photochromic bacterial photoreceptor with potential for super-resolution microscopy.
Photochem Photobiol Sci
; 12(2): 231-5, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23047813
ABSTRACT
We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin-based LOV domain, efficiently photo-switchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
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Proteínas de Bactérias
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Fotorreceptores Microbianos
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Proteínas Luminescentes
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article