Migration and differentiation of mouse embryonic stem cells transplanted into mature cochlea of rats with aminoglycoside-induced hearing loss.
Acta Otolaryngol
; 133(2): 136-43, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23050670
ABSTRACT
CONCLUSION:
Mouse embryonic stem cells (ESCs) transplanted into the scala tympani are able to migrate in the cochlea of rats deafened with aminoglycoside and partly restore the structure of sensory epithelia of the inner ear.OBJECTIVES:
To explore the migration and differentiation of enhanced green fluorescence protein (EGFP)-expressing ESCs by transplanting them into the scala tympani of rats with amikacin sulfate-induced hearing loss.METHODS:
Adult Sprague-Dawley (SD) rats were deafened with amikacin sulfate. Mouse ESCs expressing EGFP (EGFP-ESCs) were transplanted into the scala tympani. The migration and differentiation were observed at different time points.RESULTS:
EGFP-ESCs transplanted into normal cochlea did not migrate, but those in the amikacin-damaged cochlea could survive and migrate into the scala media and the vestibular cisterna. For the first time, we observed that the EGFP-ESCs migrated into the scala media, took the place of the organ of Corti, and formed a structure just like the cochlear tunnel. Some grafted stem cells even expressed myosin VIIa, the molecular marker of hair cells. Some nerve fibers reached to the bottom of the hair cell-like cells. The ESCs migrated into the vestibule and restored the sensory epithelia of the ampullary crest. The number of the transplanted ESCs reduced over the 6 week period of the study.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Rampa do Tímpano
/
Prenhez
/
Cóclea
/
Surdez
/
Transplante de Células-Tronco
/
Células-Tronco Embrionárias
/
Perda Auditiva
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article