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Characterization of CD8+ T cell function and immunodominance generated with an H2O2-inactivated whole-virus vaccine.
Walker, Joshua M; Raué, Hans-Peter; Slifka, Mark K.
Afiliação
  • Walker JM; Oregon National Primate Research Center, Oregon Health & Sciences University, Beaverton, Oregon, USA.
J Virol ; 86(24): 13735-44, 2012 Dec.
Article em En | MEDLINE | ID: mdl-23055558
CD8(+) T cells play an important role in protection against both acute and persistent viral infections, and new vaccines that induce CD8(+) T cell immunity are currently needed. Here, we show that lymphocytic choriomeningitis virus (LCMV)-specific CD8(+) T cells can be generated in response to a nonreplicating H(2)O(2)-inactivated whole-virus vaccine (H(2)O(2)-LCMV). Vaccine-induced CD8(+) T cell responses exhibited an increased ability to produce multiple cytokines at early time points following immunization compared to infection-induced responses. Vaccination with H(2)O(2)-LCMV induced the expansion of a narrow subset of the antigen-specific CD8(+) T cells induced by LCMV strain Arm infection, resulting in a distinct immunodominance hierarchy. Acute LCMV infection stimulated immunodominance patterns that shifted over time or after secondary infection, whereas vaccine-generated immunodominance profiles remained remarkably stable even following subsequent viral infection. Vaccine-induced CD8(+) T cell populations expanded sharply in response to challenge and were then maintained at high levels, with responses to individual epitopes occupying up to 40% of the CD8(+) T cell compartment at 35 days after challenge. H(2)O(2)-LCMV vaccination protected animals against challenge with chronic LCMV clone 13, and protection was mediated by CD8(+) T cells. These results indicate that vaccination with an H(2)O(2)-inactivated whole-virus vaccine induces LCMV-specific CD8(+) T cells with unique functional characteristics and provides a useful model for studying CD8(+) T cells elicited in the absence of active viral infection.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vacinas Virais / Epitopos Imunodominantes / Linfócitos T CD8-Positivos / Peróxido de Hidrogênio Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Vacinas Virais / Epitopos Imunodominantes / Linfócitos T CD8-Positivos / Peróxido de Hidrogênio Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2012 Tipo de documento: Article