Global demethylation in loss of imprinting subtype of Wilms tumor.
Genes Chromosomes Cancer
; 52(2): 174-84, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23074036
ABSTRACT
Epigenetic abnormalities at the IGF2/H19 locus play a key role in the onset of Wilms tumor. These tumors can be classified into three molecular subtypes depending on the events occurring at this locus loss of imprinting (LOI), loss of heterozygosity (LOH), or retention of imprinting (ROI). As IGF2 LOI is a consequence of aberrant methylation, we hypothesized that this subtype of Wilms tumors might display global abnormalities of methylation. We therefore analyzed the methylation status of satellite DNA, as a surrogate for global methylation in 50 Wilms tumor patients. Satellite methylation was quantified by a methylation-sensitive quantitative PCR. We confirmed hypomethylation of both satellite α (Sat α) and satellite 2 (Sat 2) DNA in Wilms tumor samples compared with normal kidney. In addition, we found that LOI tumors, unlike ROI or LOH ones, showed concordant hypomethylation of both Sat α and Sat 2 DNA. This would suggest that the LOI subtype of Wilms tumor, which unlike other subtypes results from an epimutation, has a global deregulation of methylation mechanisms.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Satélite
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Fator de Crescimento Insulin-Like II
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Tumor de Wilms
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Impressão Genômica
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Metilação de DNA
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article