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Differential responses to UVB irradiation in human keratinocytes and epidermoid carcinoma cells.
Biomed Environ Sci ; 25(5): 583-9, 2012 Oct.
Article em En | MEDLINE | ID: mdl-23122317
OBJECTIVE: To examine UVB-induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside. METHODS: Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF-κB, BCL-2, and CDK6 after 50 J/m(2) UVB irradiation were detected by RT-PCR and western blotting. RESULTS: Our results confirmed greater tolerance of A341 cells to UVB-induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF-κB, BCL-2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G(1)-S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound. CONCLUSION: Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF-κB, CDK6, and BCL-2 in UVB-induced cell G(1)-S phase arrest. Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Carcinoma de Células Escamosas / Queratinócitos Limite: Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Carcinoma de Células Escamosas / Queratinócitos Limite: Humans Idioma: En Ano de publicação: 2012 Tipo de documento: Article