The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model.
Pharm Biol
; 51(3): 298-303, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23137300
ABSTRACT
CONTEXT Silymarin, a flavonolignan from Silybum marianum (L.) Gaertn. (Asteraceae), has been reported to have antioxidant and anti-inflammatory properties. Therefore, it may be worthwhile to study the effect of silymarin on wound healing. OBJECTIVE:
To evaluate the effect of silymarin on human fibroblast cells in an in vitro model of wound healing. MATERIALS ANDMETHODS:
Human fibroblast cells were treated with different concentrations (4.5, 9, 18, 36 µg/mL) of silymarin. The effects of silymarin on cell viability, proliferation, collagen synthesis, and expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthetase (iNOS) were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, 5-bromo-2'-deoxy-uridine, hydroxyproline analysis and real-time PCR, respectively. The effect of silymarin on cellular antioxidant status was determined by protection against hydrogen peroxide (H2O2)-induced cell injury and free radical scavenging activity (ABTS assay) of the cells.RESULTS:
Results of the present study indicate that pretreatment of fibroblast cells with silymarin significantly protected cells against H2O2-induced injury (p < 0.05). After an 18 h treatment of cells with 36 µg/mL silymarin, total antioxidant capacity of cells significantly increased (p < 0.05). Furthermore, pretreatment of human fibroblast cells with silymarin significantly inhibited lipopolysaccharide (LPS)-induced COX-2 mRNA expression (p < 0.001). There was no significant difference in fibroblast proliferation and collagen synthesis between treatment and control groups (p > 0.05). DISCUSSION ANDCONCLUSION:
Silymarin may be useful as a therapeutic agent for the treatment of cutaneous wounds through its antioxidation and anti-inflammation effects.
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Base de dados:
MEDLINE
Assunto principal:
Silimarina
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Pele
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Cicatrização
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Anti-Inflamatórios não Esteroides
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Antioxidantes
Limite:
Humans
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Male
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Newborn
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article