Plasmid DNA immunization with Trypanosoma cruzi genes induces cardiac and clinical protection against Chagas disease in the canine model.
Vet Res
; 43: 79, 2012 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-23148870
ABSTRACT
The only existing preventive measure against American trypanosomosis, or Chagas disease, is the control of the transmitting insect, which has only been effective in a few South American regions. Currently, there is no vaccine available to prevent this disease. Here, we present the clinical and cardiac levels of protection induced by expression to Trypanosoma cruzi genes encoding the TcSP and TcSSP4 proteins in the canine model. Physical examination, diagnostic chagasic serology, and serial electrocardiograms were performed before and after immunization, as well as after experimental infection. We found that immunization with recombinant plasmids prevented hyperthermia in the acute phase of experimental infection and produced lymphadenomegaly as an immunological response against the parasite and additionally prevented heart rate elevation (tachycardia) in the acute and/or chronic stages of infection. Immunization with T. cruzi genes encoding the TcSP and TcSSP4 antigens diminished the quality and quantity of the electrocardiographic abnormalities, thereby avoiding progression to more severe developments such as right bundle branch block or ventricular premature complexes in a greater number of dogs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
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Proteínas de Protozoários
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Vacinas Protozoárias
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Doença de Chagas
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Vacinas de DNA
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article