Loss of CDKN2B promotes p53-dependent smooth muscle cell apoptosis and aneurysm formation.
Arterioscler Thromb Vasc Biol
; 33(1): e1-e10, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23162013
ABSTRACT
OBJECTIVE:
Genomewide association studies have implicated allelic variation at 9p21.3 in multiple forms of vascular disease, including atherosclerotic coronary heart disease and abdominal aortic aneurysm. As for other genes at 9p21.3, human expression quantitative trait locus studies have associated expression of the tumor suppressor gene CDKN2B with the risk haplotype, but its potential role in vascular pathobiology remains unclear. METHODS ANDRESULTS:
Here we used vascular injury models and found that Cdkn2b knockout mice displayed the expected increase in proliferation after injury, but developed reduced neointimal lesions and larger aortic aneurysms. In situ and in vitro studies suggested that these effects were attributable to increased smooth muscle cell apoptosis. Adoptive bone marrow transplant studies confirmed that the observed effects of Cdkn2b were mediated through intrinsic vascular cells and were not dependent on bone marrow-derived inflammatory cells. Mechanistic studies suggested that the observed increase in apoptosis was attributable to a reduction in MDM2 and an increase in p53 signaling, possibly due in part to compensation by other genes at the 9p21.3 locus. Dual inhibition of both Cdkn2b and p53 led to a reversal of the vascular phenotype in each model.CONCLUSIONS:
These results suggest that reduced CDKN2B expression and increased smooth muscle cell apoptosis may be one mechanism underlying the 9p21.3 association with aneurysmal disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Artérias Carótidas
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Proteína Supressora de Tumor p53
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Apoptose
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Aneurisma da Aorta Abdominal
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Inibidor de Quinase Dependente de Ciclina p15
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Músculo Liso Vascular
Tipo de estudo:
Observational_studies
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Prognostic_studies
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Risk_factors_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article