A germline clone screen on the X chromosome reveals novel meiotic mutants in Drosophila melanogaster.
G3 (Bethesda)
; 2(11): 1369-77, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-23173088
ABSTRACT
In an effort to isolate novel meiotic mutants that are severely defective in chromosome segregation and/or exchange, we employed a germline clone screen of the X chromosome of Drosophila melanogaster. We screened over 120,000 EMS-mutagenized chromosomes and isolated 19 mutants, which comprised nine complementation groups. Four of these complementation groups mapped to known meiotic genes, including mei-217, mei-218, mei-9, and nod. Importantly, we have identified two novel complementation groups with strong meiotic phenotypes, as assayed by X chromosome nondisjunction. One complementation group is defined by three alleles, and the second novel complementation group is defined by a single allele. All 19 mutants are homozygous viable, fertile, and fully recessive. Of the 9 mutants that have been molecularly characterized, 5 are canonical EMS-induced transitions, and the remaining 4 are transversions. In sum, we have identified two new genes that are defined by novel meiotic mutants, in addition to isolating new alleles of mei-217, mei-218, mei-9, and nod.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromossomo X
/
Mutação em Linhagem Germinativa
/
Drosophila
/
Cromossomos de Insetos
/
Meiose
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article