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[Establishment of an isolated porcine liver machine perfusion model for high-intensity focused ultrasound studies].
Bu, Rui; Zou, Jianzhong; Yin, Li; Wu, Feng; Meng, Shihe; Liu, Fang; Zhao, Xin; Jiao, Jiao; Yang, Han.
Afiliação
  • Bu R; Institute of Biomedical Engineering, Chongqing Medical University, Chongqing, China. burui0703@163.com
Nan Fang Yi Ke Da Xue Xue Bao ; 32(11): 1564-7, 2012 Nov.
Article em Zh | MEDLINE | ID: mdl-23174575
ABSTRACT

OBJECTIVE:

To explore the feasibility of establishing an isolated porcine liver machine perfusion model and assess its value in high-intensity focused ultrasound studies.

METHODS:

Twenty-one isolated porcine livers were perfused with autologous blood for 4 h through dual vessels (portal vein and hepatic artery) cannulation using an extracorporeal circulation machine under a sub-normothermic perfusion condition. The perfusion model was assessed by monitoring the liver color, texture, liver weight gain, hemodynamic parameters, color Doppler flow imaging, bile output and histopathology.

RESULTS:

Nineteen isolated porcine livers were successfully cannulated with dual vessels, and failure of hepatic artery intubation occurred in two porcine livers. After machine perfusion for 4 h, the isolated livers maintained a soft texture with stable hemodynamic levels within relative normal physiological ranges. The bile output was more than 3 ml/h within the initial 3 h of perfusion. Histopathological examination demonstrated no morphological or structural changes of the liver tissues.

CONCLUSION:

The isolated porcine liver perfusion model is stable and feasible, and can be used for high-intensity focused ultrasound studies.
Assuntos
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Base de dados: MEDLINE Assunto principal: Circulação Extracorpórea / Fígado / Circulação Hepática Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: Zh Ano de publicação: 2012 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Circulação Extracorpórea / Fígado / Circulação Hepática Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: Zh Ano de publicação: 2012 Tipo de documento: Article