[Establishment of an isolated porcine liver machine perfusion model for high-intensity focused ultrasound studies].
Nan Fang Yi Ke Da Xue Xue Bao
; 32(11): 1564-7, 2012 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-23174575
ABSTRACT
OBJECTIVE:
To explore the feasibility of establishing an isolated porcine liver machine perfusion model and assess its value in high-intensity focused ultrasound studies.METHODS:
Twenty-one isolated porcine livers were perfused with autologous blood for 4 h through dual vessels (portal vein and hepatic artery) cannulation using an extracorporeal circulation machine under a sub-normothermic perfusion condition. The perfusion model was assessed by monitoring the liver color, texture, liver weight gain, hemodynamic parameters, color Doppler flow imaging, bile output and histopathology.RESULTS:
Nineteen isolated porcine livers were successfully cannulated with dual vessels, and failure of hepatic artery intubation occurred in two porcine livers. After machine perfusion for 4 h, the isolated livers maintained a soft texture with stable hemodynamic levels within relative normal physiological ranges. The bile output was more than 3 ml/h within the initial 3 h of perfusion. Histopathological examination demonstrated no morphological or structural changes of the liver tissues.CONCLUSION:
The isolated porcine liver perfusion model is stable and feasible, and can be used for high-intensity focused ultrasound studies.
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Base de dados:
MEDLINE
Assunto principal:
Circulação Extracorpórea
/
Fígado
/
Circulação Hepática
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article