Cell cycle analysis of the CD133(+) and CD133(-) cells isolated from human colorectal cancer.
J Cancer Res Ther
; 8(3): 399-403, 2012.
Article
em En
| MEDLINE
| ID: mdl-23174722
ABSTRACT
AIM:
The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors. MATERIALS ANDMETHODS:
After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells.RESULTS:
The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase.CONCLUSION:
The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Células-Tronco Neoplásicas
/
Glicoproteínas
/
Neoplasias Colorretais
/
Antígenos CD
/
Ciclo Celular
/
Neoplasias Hepáticas
Limite:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article