Role of LOXs and COX-2 on FAK activation and cell migration induced by linoleic acid in MDA-MB-231 breast cancer cells.
Cell Oncol (Dordr)
; 36(1): 65-77, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23179791
ABSTRACT
BACKGROUND:
Epidemiological studies and animal models suggest a link between high levels of dietary fat intake and an increased risk of developing breast cancer. Particularly, free fatty acids (FFAs) are involved in several processes, including proliferation, migration and invasion, in breast cancer cells. Linoleic acid (LA) is a dietary n-6 polyunsaturated fatty acid that is known to induce proliferation and invasion in breast cancer cells. So far, however, the contribution of LA to focal adhesion kinase (FAK) activation and cell migration in breast cancer cells has not been studied.RESULTS:
Here, we show that LA promotes FAK and Src activation, as well as cell migration, in MDA-MB-231 breast cancer cells. FAK activation and cell migration require Src, Gi/Go, COX-2 and LOXs activities, whereas both are independent of Δ6 desaturase activity. In addition, we show that cell migration requires FAK activity, whereas FAK activation requires Src activity, thus suggesting a reciprocal catalytic activation mechanism of FAK and Src.CONCLUSIONS:
In summary, our findings show that LA induces FAK activation and cell migration in MDA-MB-231 breast cancer cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Movimento Celular
/
Ácido Linoleico
/
Ciclo-Oxigenase 2
/
Proteína-Tirosina Quinases de Adesão Focal
/
Lipoxigenases
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article