Rosamine-based fluorescent sensor with femtomolar affinity for the reversible detection of a mercury ion.
Inorg Chem
; 51(24): 13075-7, 2012 Dec 17.
Article
em En
| MEDLINE
| ID: mdl-23186173
ABSTRACT
A Hg(2+)-selective fluorescent sensor, RosHg, has been developed based on a rosamine platform. RosHg exhibited a â¼20-fold increase in fluorescence emission upon binding with Hg(2+), and the enhanced fluorescence was immediately decreased when glutathione was added to a solution of the Hg-RosHg complex. The dissociation constant for the Hg(2+) complex was determined to be 0.10 fM by using a set of Hg(2+)/Mg(2+)/ethylenediaminetetraacetic acid buffer solutions. Confocal microscopy experiments demonstrated that this sensor can monitor changes of the Hg(2+) level in the mitochondria of living cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Corantes Fluorescentes
/
Mercúrio
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article